Full Record - Images of histological sections of oocytes for the bivalves Astarte crenata and Bathyarca glacialis from the Western Barents Sea under ambient and near-future climate change conditions.

Images of histological sections of oocytes for the bivalves Astarte crenata and Bathyarca glacialis from the Western Barents Sea under ambient and near-future climate change conditions.

GB/NERC/BAS/PDC/01564

Abstract:
Images of histological sections of oocytes to quantify the interactive effects of ocean warming and ocean acidification based on near-future climate change projections on oocyte size frequency distributions of benthic invertebrates (the bivalves Astarte crenata and Bathyarca glacialis) from the Western Barents Sea.

Supported by The Changing Arctic Ocean Seafloor (ChAOS) - how changing sea ice conditions impact biological communities, biogeochemical processes and ecosystems project (NE/N015894/1 and NE/P006426/1, 2017-2021), Natural Environment Research Council (NERC) in the UK.

Citation:
Reed, A., Godbold, J., Solan, M., & Grange, L. (2021). Images of histological sections of oocytes for the bivalves Astarte crenata and Bathyarca glacialis from the Western Barents Sea under ambient and near-future climate change conditions. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/04766a75-502c-4431-b771-107326a8585c

Access Data

License

Use:	This data is governed by the NERC data policy http://www.nerc.ac.uk/research/sites/data/policy/ and supplied under Open Government Licence v.3 http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/

Authors

Name	UK Polar Data Centre
Role(s)	Metadata Author
Organisation	British Antarctic Survey

Name	Adam J Reed
Role(s)	Investigator
Organisation	University of Southampton

Name	Jasmin A Godbold
Role(s)	Investigator
Organisation	University of Southampton

Name	Martin Solan
Role(s)	Investigator
Organisation	University of Southampton

Name	Laura J Grange
Role(s)	Investigator
Organisation	Bangor University

Basic Information

Creation Date:	2021-09-17
Dataset Progress:	Planned
Dataset Language:	English
ISO Topic Categories:	Biota
Parameters:	Biosphere > Ecological Dynamics > Life History Oceans > Marine Biology > Marine Invertebrates

Additional Information

Quality:	Standard protocols were followed and data entry double checked by independent person.
Lineage/Methodology:	Specimens were collected from each of 1 station in 2017 (B13) and 2 stations in 2018 (B16 and B17) during two consecutive cruises (RRS James Clark Ross: JR16006, 30th June to 8th August 2017; JR17007: 10th July to 5th August, 2018) following a transect along the 30°E meridian. At each station similar-sized specimens of the infaunal bivalves Astarte crenata and Bathyarca glacialis were obtained by Agassiz trawl. Surficial sediment < 10 cm deep was collected using SMBA box cores, sieved to remove macrofauna (500 µm mesh), and homogenised by stirring. Both infaunal bivalves and sediment were maintained under aerated seawater and returned to the Biodiversity and Ecosystem Futures Facility, University of Southampton at ambient temperature (1.5 + 1°C). Ten centimetres of homogenised sediment were transferred to transparent acrylic aquaria (internal dimensions, LWH: 20 x 20 x 34 cm) and overlain with ~8 L (20cm depth) surface seawater (salinity, ~34). Aquaria were maintained in the dark at ambient conditions (1-2 °C and ~400 ppm [CO2]), and randomly distributed between two insulated fibreglass seawater baths (LWH: 1.2 x 1.2 x 0.8m, Tanks Direct, UK). Three B. glacialis were introduced to each of twelve aquaria (n = 36; ambient 18, future 18) and six A.crenata were introduced to each of ten aquaria (n = 60; 30 ambient, 30 future). After a 30-day acclimation period to aquarium conditions, temperature and [CO2] were manually adjusted (1 °C and ~100 ppm CO2 week -1) to achieve near-future treatment conditions (3-5 °C and ~550 ppm [CO2]). We periodically measured pH, temperature and salinity and total alkalinity. The bivalves were fed ad libitum three time per week (100 ml cultured live Isochrysis sp., Tetraselmis sp., and Phaeodactylum sp.), and overlying water exchanged weekly. Experiments were run for 120 days (B. glacialis, 21//11/2017-20/03/2018) or 135 days (A. crenata, 08/10/2018-19/02/2019) after which animals were removed and fixed in 4 neutral buffered formaldehyde. Oocyte images: Images of stained slides of histological sections from individual females were captured using a Nikon D5000 digital SLR camera mounted on an Olympus (BH-2) stereomicroscope and analysed using ImageJ v 1.48. Slides were photographed at X10 magnification. Replicate photographs were taken to ensure 100 unique oocytes were measured for each female. Composite images were generated for easy analysis wherever possible. Full descriptions and additional information provided in Reed et al. 2021 Frontiers in Marine Science in main manuscript and supplementary information.

Extent

Temporal Coverage:
Start Date	2017-07-22
End Date	2017-08-01

Start Date	2018-07-19
End Date	2018-07-28

Spatial Coverage:
Latitude
Southernmost	74.49999
Northernmost	81.28249
Longitude
Westernmost	29.23142
Easternmost	30.50174
Altitude
Min Altitude	N/A
Max Altitude	N/A
Depth
Min Depth	280
Max Depth	359

Latitude
Southernmost	74.50008
Northernmost	81.28136
Longitude
Westernmost	29.32781
Easternmost	30.50113
Altitude
Min Altitude	N/A
Max Altitude	N/A
Depth
Min Depth	228
Max Depth	360

Location:
Location	Arctic
Detailed Location	Western Barents Sea

Instrumentation

Data Collection:	Resolution: Biometric measurements (i.e., shell length, shell height and tumidity (width) to two decimal places. Wet weight to four decimal places. Oocyte Equivalent Circular Diameter to two decimal places. Instrumentation: Mettler-Toledo InLab Expert Pro temperature-pH combination electrode Mettler-Toledo InLab 737 IP67 temperature-conductivity combination electrode Digital vernier calliper Balance (+/-0.000g) Rotary microtome Nikon D5000 digital SLR camera Olympus BHS (BH-2) stereomicroscope

Data Collection:

Resolution:
Biometric measurements (i.e., shell length, shell height and tumidity (width) to two decimal places.
Wet weight to four decimal places.
Oocyte Equivalent Circular Diameter to two decimal places.

Instrumentation:
Mettler-Toledo InLab Expert Pro temperature-pH combination electrode
Mettler-Toledo InLab 737 IP67 temperature-conductivity combination electrode
Digital vernier calliper
Balance (+/-0.000g)
Rotary microtome
Nikon D5000 digital SLR camera
Olympus BHS (BH-2) stereomicroscope

Storage

Data Storage:	Folder names: cruisenumber_species_exp_spcode N.B For species, Ast represents Astarte crenata and Bathy represents Bathyarca glacialis. exp = climate experiment. spcode = specimen code = individual number/ identifier. Each folder contains a number of subfolders representing the slide number from which the contained images are taken. Subfolders where the slide number prefix is recorded to 1 d.p. indicate the position on the slide where the contained image(s) were taken. This position was arbitrarily assigned by the individual undertaking image analysis and was needed when multiple sections of tissue were mounted onto one slide. This step was taken in an effort to keep track of which section was being imaged. Note: For individuals where fewer images were taken, multiple subfolders identifying 'slide number' were not required. These images are archived individually in the main specimen code folder. A small 'c' represents images that were taken as a composite image of an entire histology section (and therefore will overlap). However, owed to the fact that it was not always possible to render a completed composite image accurately, a complete composite image is not available in every folder labelled 'c'. The notation "nm" or "not measured" indicates images where the oocytes photographed were not measured.

Data Storage:

Folder names: cruisenumber_species_exp_spcode N.B For species, Ast represents Astarte crenata and Bathy represents Bathyarca glacialis. exp = climate experiment. spcode = specimen code = individual number/ identifier.

Each folder contains a number of subfolders representing the slide number from which the contained images are taken. Subfolders where the slide number prefix is recorded to 1 d.p. indicate the position on the slide where the contained image(s) were taken. This position was arbitrarily assigned by the individual undertaking image analysis and was needed when multiple sections of tissue were mounted onto one slide. This step was taken in an effort to keep track of which section was being imaged. Note: For individuals where fewer images were taken, multiple subfolders identifying 'slide number' were not required. These images are archived individually in the main specimen code folder.

A small 'c' represents images that were taken as a composite image of an entire histology section (and therefore will overlap). However, owed to the fact that it was not always possible to render a completed composite image accurately, a complete composite image is not available in every folder labelled 'c'.
The notation "nm" or "not measured" indicates images where the oocytes photographed were not measured.